CHEM 773-7730-02 - Fluorescence: Principles, Theory, and Applications to the Characterization of Macromolecules

Semester: Fall 2020

Professor: J. Duhamel | Discipline: Polymer |


The course will cover the fluorescence phenomenon, the steady-state and time-resolved fluorometers, the solvent effects on dyes, fluorescence quenching, fluorescence resonance energy transfer (FRET), fluorescence anisotropy, analysis of fluorescence decays, and as special topics, some applications of fluorescence based in part on the amazing photophysical properties of pyrene. If time permits, the course will also expand to confocal fluorescence microscopy and fluorescence correlation spectroscopy.


Principles of Fluorescence Spectroscopy (3rd Edition) by Joseph R. Lakowicz (Springer: NY, 2006; DOI: 10.1007/978-0-387-46312-4)


In class mid-term exam (40-60%) on Monday October 20th, 2020, and a 21/2-hour final exam (40-60%).




  • Tue: 7:00 pm - 9:20 pm in EIT 2053/MacN 101

Office Hours

Room: C2-163; Tel: 35916; E-mail: [email protected] Office hours: On demand