CHEM 731-7310-01 - Biomolecular NMR Spectroscopy of Proteins and Nucleic Acids
Semester: Fall 2024
Professor: T. Dieckmann | Discipline: Biochemistry | Campus: WaterlooDescription
Discussion of specialized topics related to the research interests of the members of the Centre. For example, recent offerings have included: Metalloproteins and Metalloenzymes; Heme Proteins; Chemistry of Enzymatic Reaction Mechanisms; Peptides – Synthesis, Structure and Function; Food Enzymology; Advanced Microbial Physicology; NMR in Biological Systems.
This course covers the basics of biomolecular NMR spectroscopy and its application to protein and nucleic acid structure determination.
Among the topics discussed are:
- Basics of NMR spectroscopy
- Spectrometer hardware
- Types of pulse sequences and their application
- Extracting NMR parameters from spectra
- Types and use of software for the analysis of NMR spectra and structure calculation/analysis
- Preparation and writing of a biomolecular NMR based research paper
Learning Outcomes
By the end of this course students should be able to:
| develop an understanding of biomolecular NMR spectroscopy and its applications |
| develop an up-to-date knowledge of methods used to derive protein structures from NMR data in both, theory and practice |
| gather experience in analyzing NMR data and preparing the results for publication. |
Materials
No materials required.
All material needed will be provided in class and via the class website. This includes software and data needed for the practical portion of the course. Access to a computer (in class, at home or in a campus computer lab is required to complete the project)
Evaluation
| Component | Value |
| Mid-term Examination (Nov. 23rd) | 40 |
| Term Paper (due on December 3rd) | 60 |
Rules for Group Work in Lab Assignments: All work on the midterm and term paper must be performed by each student on their own .
Lab/Project
Lectures for the first 8 weeks followed by work on the term project.
Schedule
- Wed: 7:00 pm - 9:20 pm in C2 278
