CHEM 731/7310-01 - Biomolecular NMR

Semester: Fall 2021

Professor: T. Dieckmann | Discipline: Biochemistry | Campus: Waterloo


This course covers the basics of biomolecular NMR spectroscopy and its application to protein and nucleic acid structure determination.

Among the topics discussed are:

• Basics of NMR spectroscopy
• Spectrometer hardware
• Types of pulse sequences and their application
• Extracting NMR parameters from spectra
• Types and use of software for the analysis of NMR spectra and structure calculation/analysis
• Preparation and writing of a biomolecular NMR based research paper.

Course Objectives:

• Students will develop an understanding of biomolecular NMR spectroscopy and its applications.
• Students will develop an up-to-date knowledge of methods used to derive protein structures from NMR data in both, theory and practice.
• Students will gather experience in analyzing NMR data and preparing the results for publication.


All material needed will be provided in class and via the class website. This includes software and data needed for the practical portion of the course. Access to a computer (in class, at home or in a campus computer lab is required to complete the project).


The course grade will be based on one mid-term examination at the end of the 6 weeks
basic section (Tuesday, November 2nd, 2021) and a term paper (Due on December 10th , 2021. The breakdown is as follows:

Mid-term Examination 40% Tuesday, November 2nd 2021
Term Paper 60% Due on Tuesday, December 10th, 2021


Rules for Group Work in Lab Assignments: All work on the midterm and term paper must be performed by each student on their own.

Late Submissions: Late submission of the term paper will be accepted until December 12th. Late submissions will have 25% of the mark deducted for each day or part of a day that the assignment is late. Assignments submitted after December 12th will receive a mark of 0%.


  • Tue: 7:00 pm - 9:20 pm in C2 278/MacN 203

Office Hours

ESC 227, by appointment, [email protected]; ext 35036